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Figure 1


Fig. 1. Ncd in the meiotic and mitotic spindle. (A) Ncd constructs. FLNcdVenus consists of an N-terminal basic, proline-rich tail, {alpha}-helical coiled-coil stalk (grey) and conserved motor domain or head, with the Venus fluorescent protein (green) at the C terminus. HLNcdVenus is deleted for the head and TLNcdVenus, for the tail. (B) FLncdVenus; cand oocyte meiosis I spindle (top), abnormal multi-polar and multiple small HLncdVenus; cand meiosis I spindles (middle) and absence of TLNcdVenus fluorescence in a TLncdVenus; ncdmRFP cand meiosis I spindle (bottom). Scale bars: 5 µm. (C) Prometaphase to telophase in a HLncdVenus cycle 10 (left) and TLncdVenus cycle 9 (right) mitotic division. Abnormal fused, spurred, bridged or bent spindles, or failure of midzone to form (arrows). Time, minutes:seconds. Scale bar: 10 µm.