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Figure 3


Fig. 3. NcdVenus fluorescence recovery in the mitotic spindle. (A) FLncdVenus spindle FRAP assays at the equator in a small (radius, w=1.3 µm) (top) and large (w=2.66 µm) (bottom) ROI. (B) Mean fluorescence recovery curves (magenta; small ROI, n=5; large ROI, n=11) after normalization to the first prebleach image and correction for fluorescence loss during recovery imaging. Inset, curve fits to the new diffusion-binding-transport model (dark green). (C) HLncdVenus spindle FRAP assays at the equator in a small (w=1.3 µm) (top) and large (w=2.66 µm) (bottom) ROI. (D) Mean fluorescence recovery curves (purple; small ROI, n=8; large ROI, n=10) after normalization and correction for loss during imaging. Inset, curve fits to the new diffusion-binding-transport model (dark green). (E) TLncdVenus spindle FRAP assays at the equator in a small (w=1.3 µm) (top) and large (w=2.66 µm) (bottom) ROI. (F) Mean fluorescence recovery curves (violet; small ROI, n=8; large ROI, n=11) after normalization and correction for loss during imaging. Inset, curve fits to the diffusion-binding model (Sprague et al., 2004) (dark green). Scale bars: 3 µm.