Fig. 8. Preferential transmigration of EphBR-expressing monocytes through ephrin-B2-expressing EC monolayers. Mock-transduced cells as well as ephrin-B2- and C-ephrin-B2-overexpressing HUVEC were grown to confluence on gelatine-coated membrane filters. Confluence was monitored by calcein staining (top). J774 cells were allowed to transmigrate for 24 hours towards FCS. Quantification of transmigrated J774 cells revealed the preferential transmigration of J774 cells through ephrin-B2-overexpressing HUVEC monolayers but not through C-ephrin-B2-overexpressing or mock-transduced HUVEC monolayers (center). Representative images show PKH26-labeled transmigrated J774 cells on the lower side of the Transwell membrane (bottom). Values are expressed as the mean ± s.d. of one out of three independent experiments with similar results (*P<0.05). Scale bars: 500 µm.