Fig. 4. unc-94/tmd-1(RNAi) strongly enhances actin disorganization in an unc-78-null background. (A) Localization of TMD-1 and UNC-78 in unc-78 mutant and unc-94/tmd-1(RNAi) muscles, respectively. TMD-1 (a,d) and actin (b,e) were immunolocalized in the body-wall muscle of wild-type (a-c) and unc-78(gk27) (d-f). Merged images are shown in c and f (TMD-1, green; actin, red). UNC-78 (g,j) and actin (h,k) were immunolocalized in the body-wall muscle of wild-type (g-i) and unc-94/tmd-1(RNAi) (j-l). Merged images are shown in i and l (UNC-78 in green and actin in red). (B) Effects of unc-94/tmd-1(RNAi) on worm motility in an unc-78 null background. Wild-type and unc-78(gk27) were examined. Data are the mean ± s.d., n=10. Asterisks indicate P<0.005 by t-test comparing control RNAi experiments and unc-94/tmd-1(RNAi) for each strain. (C) Appearance of wild-type (a,c) and unc-78(gk27) (b,d) worms treated with control dsRNA (a,b) or unc-94/tmd-1(RNAi) (c,d) on agar plates. (D) Actin filament organization in the body-wall muscle of wild-type (a,b) and unc-78 (c,d) treated with control dsRNA (a,c) or unc-94/tmd-1(RNAi) (b,d).