Fig. 1. (A) Summary of cellular changes that take place during initiation of follicle growth. (B) Stages of follicle development analysed in this study. (C) Ovary section stained with H&E, showing examples of follicles at primordial (p), transitional (t) and primary-plus stage. In the primary-plus follicle the majority of GCs are columnar and form a single layer (GCs), although there are some areas that start to show signs of multilayering (e.g. boxed area). Note basement membrane (white arrowheads), theca cells (black arrowheads) and GCs undergoing mitosis (black arrow). Scale bar: 20 µm.