Fig. 1. Eg5 interacts with Nek6 and Nercc1. (A) Immunoprecipitates (IP) were prepared from exponentially growing (Exp) or nocodazole-arrested mitotic (M) U2OS cell extracts using either normal rabbit IgG (NIgG), anti-Nek6 or anti-Nercc1 antibodies and analyzed by immunoblot (W) with the indicated antibodies. Eg5 in the corresponding extracts is shown in the lower panel. (B) Immunoprecipitates were prepared from exponentially growing or nocodazole-arrested mitotic U2OS cells using NIgG or anti-Nercc1 antibody, and immunoblotted for Nek6 and Nercc1.