Fig. 6. Cooperative binding of the Mto1/2 complex to
-TuC. Purification of TAP-tagged Mto1 and Mto2 in wild-type and mutant backgrounds (strains KS3524, KS3956, KS4323, KS4326, KS516, KS4267, KS4270), probed with antibodies to the indicated proteins. Differently migrating forms of Mto1 and Mto2 represent TAP-tagged, cleaved TAP-tagged, and untagged forms of the proteins. Vertical lines indicate blank lanes.