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Figure 2


Fig. 2. RUNX1/AML1 and AML1-ETO associate with metaphase chromosomes in pairs. (A) Epitope-tagged RUNX1/AML1 and AML1-ETO were examined by in situ immunofluorescence in HeLa cells and metaphase cells were visually selected. RUNX1/AML1 and AML1-ETO foci (green) are associated with chromosomes (blue) in pairs (shown in deconvoluted images). (B) Mitotic chromosome spreads were prepared for human Jurkat, HEL and Kasumi-1 cells and processed for immunofluorescence microscopy using antibodies directed against the RUNX1/AML1, ETO and AML1-ETO proteins. RUNX1/AML1 and AML1-ETO show distinct foci on the mitotic chromosome spreads, whereas ETO is not detected on mitotic chromosomes. Panels above each image show enlargements of the areas labeled with numbers.