Fig. 4. Depletion of AP-2 subunits perturbs endocytic trafficking of Arf6-pathway cargoes. Live cells depleted of -adaptin (left) or µ2-subunit (middle) were incubated with antibody against β1 integrin. Samples were fixed and stained with -adaptin antibody (top) to identify depleted cells. Right, live -adaptin-depleted cells were incubated with antibody against MHCI. Cells were fixed and stained with -adaptin antibody. Arrows indicate depleted cells.