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Figure 4


Fig. 4. MMS-induced effects on cell-cycle progression. (A) Flow cytometry histograms of G1-synchronised wild-type (left) and gcn2 (right) control cells (shaded) and cells treated with 0.15% MMS (unshaded) incubated for the times indicated after MMS treatment. (B-E) Immunoblots (for details, see Fig. 1) of wild-type (B) and gcn2 (D) cells probed with antibody against Rum1. Immunoblots of wild-type (C) and gcn2 (E) cells probed with antibody against phosphorylated Cdc2. Antibody against tubulin was used as loading control. Quantifications are shown as white columns for the control and shaded columns for MMS-treated cells.