Fig. 10. Kinetics of stress-induced Calcofluor-white staining patterns in mutant cells with constitutively activated (black line) or constitutively downregulated (red line) Plo1 kinase. Wild-type, plo1.S402A and plo1.S402E cells were grown and processed as described in Fig. 1A, and the appearance of SICS at cell tips was plotted as a function of time.