Fig. 9. SICS, tip growth and cell morphology in sorbitol-treated cells lacking the polarity factors Tea1 or Wsh3. (A-G) Wild type, tea1. and wsh3. cells were either processed as described for Fig. 1A to determine the kinetics of the appearance of SICS at cell tips (A,B,E) and to analyze cell morphology (C,D,E), or processed with fluorescent lectin for the analysis of tip growth as described for Fig. 4C (F,G). The arrow in the right-hand panel of E indicates a wsh3. cell with SICS at one tip (the end that was growing prior to the addition of sorbitol) that has re-initiated growth at a site on the cell cortex. (F) Quantification of the kinetics of the appearance of cells with dark tip regions in lectin-coated exponentially growing strains following sorbitol treatment. Note that tea1. and wsh3. cells re-initiate growth with the same kinetics as wild-type cells. (G) Pulsed lectin staining of wsh3. cells. Top panel, Calcofluor; middle, lectin; lower, merged (lectin in green, Calcofluor in red). The highlighted cell (star) has SICS at one tip (the growing tip prior to the addition of sorbitol) and has re-initiated tip growth (dark non-lectin-stained region) at the opposite (previously non-growing) tip. (H) tea1.pkGFP cells were grown to mid-log phase in YES media, fixed and processed for anti-Pk immunofluorescence either immediately before stress (upper panel) or 20 minutes post-stress (lower panel). (I) Maximum projections of wsh3.tdTomato cells either immediately before stress (upper panel) or 21 minutes after the addition of sorbitol to a final concentration of 1.2 M (lower panel). Scale bars: 5 µm.