Fig. 3. Role of activated Src by HCH in the number of gap junction plaques and functionality. 42GPA9 Sertoli cells transfected with Cx43-GFP were incubated in the presence or absence of HCH for different time periods (0-30 minutes), with or without PP2 or PD98059. (A) The number of cells with Cx43-GFP gap junction plaques was quantified under each condition and expressed in percent vs total transfected cells identified by the presence of GFP at the plasma membrane and within the cytoplasm. (B) Dye coupling analysis, as described in Materials and Methods, in control and HCH-treated 42GPA9 Sertoli cells. Notice that calcein fluorescence has noticeably recovered in control cells but not in HCH-exposed cells. (C) Dye-transfer efficiency is expressed as percentage of fluorescence recovery after calcein photobleaching in Sertoli cells cultured for the same time periods (0-30 minutes) in the presence of HCH with or without PP2 or PD98059. ^*P<0.05 and ^**P<0.01, significantly different from untreated cells.