Fig. 4. Subcellular localization of Cx43 and Src in response to HCH exposure. Sertoli cells were treated with 50 µM HCH for 10 minutes in the presence or absence of 30 µM PP2 or 30 µM PP3. Cx43 and Src were then detected by immunofluorescence. Notice that HCH treatment resulted in both aberrant cytoplasmic localization of Cx43 and recruitment of Src to the plasma membrane (arrows), is totally prevented by the presence of PP2 but not of PP3. In both situations, proteins relocation are easily noticeable by membranous lines (arrows) or cytoplasmic dots (arrowheads). Images are a typical example of three separate experiments.