Fig. 2. Both the BUZ domain and the first catalytic domain (CAT1) of HDAC6 are able to bind FAT10. (A) HEK293T cells, transfected with HA-FAT10 and the indicated FLAG-HDAC6 mutants, were treated with 5 µM MG132, or DMSO as a negative control, for 6 hours. Cell lysates were subjected to anti-FLAG immunoprecipitation (IP) and analysis by western blot (WB). Asterisks denote the position of antibody light chains. (B) Schematic representation of the mutants used in A. CAT1, first catalytic domain; CAT2, second catalytic domain; BUZ, ubiquitin-binding zinc finger.