Fig. 6. The model conjugate FAT10-GFP localizes to aggresomes under proteasome inhibition. (A) HEK293T cells transfected with FLAG-HDAC6, GFP or FAT10-GFP were treated with 5 µM MG132 or DMSO for 6 hours and immunostained with anti-GFP antibody (green), anti-FLAG antibody (red) and DAPI (blue). Scale bars: 5 µm. (B) Quantification of the percentage of cells with GFP-containing aggresomes, n100. Error bars represent the standard error of the mean (s.e.m.); statistical analysis was carried out using Student's t-test.