Fig. 7. Localization of FAT10 to aggresomes is dependent on the tubulin network and on HDAC6. (A) HEK293T cells transfected with HA-FAT10 were treated with 5 µM MG132, 1 µM nocodazole or mock treated for 6 hours, and immunostained with anti-HA antibody (green) and DAPI (blue). (B) HDAC6 wild-type (a-h) or knock-out (i-p) cells were transiently transfected with HA-FAT10 followed by treatment with 5 µM MG132 or DMSO for 4 hours and immunostained with anti-HA antibody (green), anti-ubiquitin antibody (red) and DAPI (blue). Scale bars: 5 µm.