Fig. 5. Accumulation of p53 in the nucleolus in human bladder and lung carcinomas. Lung and bladder carcinoma tissues stained for p53 by immunohistochemistry (A) and co-stained for p53 and UBF by immunofluorescence (B). Immunohistochemistry and immunofluorescence were performed as described in Materials and Methods. Red arrows indicate cells with nucleolar accumulation of p53 and black arrows indicate no nucleolar association of p53.