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Figure 2


Fig. 2. RT-PCR analysis of splicing products. (A) Schematic representation of the SUMO1 gene with various primer sets (arrows) used to detect (1) the normal spliced transcript; (2) the transcript splicing into the gene trap vector; (3) the transcript skipping the vector and truncated exon 2. (B) Agarose gel showing RT-PCR products generated using the primer sets shown in A and RNA derived from E15.5 embryos of the indicated genotype. het, heterozygous; hmz, homozygous. The lane numbers correspond to the primer sets in A. m, 100 bp ladder molecular size marker.