Fig. 5. EB1 comet intensity fluctuates in mor1-1 mutants at 21°C and is greatly reduced at 31°C. (A,B) Transgenic ProEB1::EB1-GFP line (in wild-type background) at 21°C (A) and 31°C (B). (C,D) mor1-1 expressing ProEB1::EB1-GFP at 21°C (C) and 31°C (D). Time-lapse images of ProEB1::EB1-GFP were collected as described in Fig. 4 but at 8 second intervals. To compare fluorescence between treatments, images were collected from the same cell with identical confocal settings before and after the temperature increase. The same contrast adjustments were applied to the images obtained from wild type and mor1-1. Arrowheads follow one comet between time frames in each series. Comets in mor1-1 at 31°C (D) were less abundant and rarely persisted for several time frames (upper arrowheads) or were detected at only one time point (lower arrowhead) within a series. A-D correspond to Movies 5-8 in supplementary material. Bars, 5 µm.