Fig. 6. EB1-GFP associates with microtubule side walls in mor1-1 at 31°C. Changes in GFP-EB1 distribution patterns are shown for the same cells before and after the temperature increase using identical confocal settings and contrast adjustments. (A,B) Transgenic ProEB1::EB1-GFP in wild type at 21°C (A) and 31°C (B). Side wall labelling was not detected in wild type at 31°C. (C,D) mor1-1 expressing ProEB1::EB1-GFP at 21°C (C) and 31°C (D). At 31°C, side wall labelling (arrowheads) became obvious in mor1-1. Bars, 5 µm.