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Figure 1


Fig. 1. Effect of pharmacological or genetic inactivation of class IA PI3K isoforms on CSF1-induced phosphorylation of Akt. Upper panel, WT BMMs were pre-treated for 1 hour with PW12 (0.5 µM), TGX155 (0.5 µM) or IC87114 (5 µM), followed by incubation with CSF1 (30 ng/ml) for 10 minutes and analysis of phosphorylation of Akt (on T308 and S473) by western blotting of total cell lysates (80 µg/lane). Graph represents the mean ± s.e.m. of a representative experiment performed in triplicate (**P<0.01). Lower panel, BMMs with heterozygotic inactivation of p110{alpha} or p110{delta} were stimulated with 30 ng/ml CSF1 for the indicated time points, followed by analysis as described above. Graphs represent the mean ± s.e.m. of three separate experiments (*P<0.05).