Fig. 6. Effect of pharmacological inactivation of class IA PI3K isoforms on CSF1-induced phosphorylation of Akt in macrophage cell lines. Cells were pre-treated for 1 hour with PW12 (0.5 µM), TGX155 (0.5 µM) or IC87114 (5 µM), followed by incubation with CSF1 (30 ng/ml) for 10 minutes and analysis of phosphorylation of Akt (on T308 and S473) by western blotting of total cell lysates (80 µg/lane). Graph represents the mean ± s.e.m. of two experiments performed in duplicate (^*P<0.05; ^**P<0.01; ^***P<0.001).