Fig. 7. (A) Expression levels of class IA PI3K isoforms in BAC1.2F5 cells and BMMs (WT or expressing inactive p110 or p110) were determined by immunoblotting of total cell lysates (80 µg per lane). (B) Selective recruitment of p110 to the CSF1R in BMMs but not in BAC1.2F5 cells. WT BMMs or BAC1.2F5 cells were incubated with CSF1 (30 ng/ml) for the indicated times, followed by lysis and immunoprecipitation of p110 isoforms. The co-precipitated CSF1R was detected by western blotting. Equal loading was assessed by reprobing the membrane with antibodies against p85. TCL, total cell lysate (100 µg). For BMMs, a representative experiment out of three is shown. For BAC1.2F5 cells, the results of two independent, representative experiments are shown.