Fig. 6. Intracellular localization of TNGW1 with other GWB components. (A) Both TNGW1 and GW182 colocalized to GWBs together with transfected Ago2. HeLa cells transfected with either GST-tagged GW182 or GST-tagged TNGW1 (red) and EGFP-Ago2 (green) were fixed and analyzed by indirect immunofluorescence 24 hours after transfection. Both TNGW1 and GW182 formed cytoplasmic foci and colocalized with Ago2. Bar, 10 µm. (B) Transfected TNGW1 colocalized with RNA decay factors enriched in GWBs. HeLa cells transfected with EGFP-TNGW1 were fixed 24 hours after transfection and analyzed by indirect immunofluorescence assay using rabbit anti-Dcp1a (red) and human serum IC6 (magenta, recognizes Ge-1, RAP55 and unrelated nuclear envelope protein). The transfected EGFP-TNGW1 formed cytoplasmic foci that were co-stained using both anti-Dcp1a and IC6 antibodies. Bar, 10 µm.