Fig. 7. Knockdown of TNGW1 has no apparent effect on the assembly of GWBs in HeLa cells. HeLa cells were transfected with siTNR or siGW182 and then harvested at 0, 1, 2 or 3 days after transfection. Indirect immunofluorescence assay was performed by using rabbit anti-Dcp1a (red) and human anti-GWB serum 18033 (green) to examine the effect of either siRNA on GWB formation. Bar, 10 µm.