Fig. 1. PLD is upregulated during C2C12 differentiation. Confluent C2C12 cells were induced to differentiate by serum withdrawal. Cells were lysed every 24 hours, and the lysates were subjected to (A) quantitative RT-PCR and (B) western analysis. (C) In vivo transphosphatidylation assays were performed with differentiating C2C12 cells. The average results of three independent experiments are shown, with error bars representing s.d. Student's t-test was performed to compare the data from day 1 to 3 with that of day 0. ^*P<0.05; ^**P<0.01.