Fig. 2. 1-butanol inhibits C2C12 differentiation. C2C12 myoblasts were induced to differentiate for 3 days in the presence or absence of 0.5% 1-butanol, 0.5% 2-butanol, or 50 nM rapamycin. (A) The cells were immunostained for MHC (green) and DAPI (red). (B) Fusion index of cells shown in A. (C) Cells were lysed and subjected to western analysis for MHC, myogenin and tubulin. Three independent experiments were performed with similar outcomes; the result of a representative experiment is shown.