Fig. 4. gp210 is enriched in the NE and its remnants during NEBD. Wild-type embryos were fixed, processed for immunostaining for gp210 (green in merge) or NPCs (using mAb414, red in merge) together with Hoechst chromatin staining (blue in merge) and observed by confocal microscopy. gp210 remained associated with the mitotic NE (arrowheads in metaphase and anaphase), whereas NPCs were largely disassembled (mAb414). The intensity of the gp210 signal was even stronger during metaphase than during interphase or telophase. Bar, 10 µm.