JCS -- Chidambaram et al. 121 (3): 329 Figure IG2

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Fig. 2. Genetic interactions between SYN8, ENT3 and ENT5. (A) The absence of Ent3p and Ent5p led to a synthetic growth defect in syn8 ${Delta}$ cells at 30°C and 37°C. (B) Absence of Ent3p and Ent5p resulted in synthetic defects of CPY transport to the vacuole in syn8 ${Delta}$ cells. CPY was immunoprecipitated from cellular extracts (I) and the medium (E) after pulse-chase labeling at 30°C. p1CPY, endoplasmic reticulum pro1CPY; p2CPY, Golgi pro2CPY; mCPY, vacuolar mature CPY. (C) Quantification of CPY secretion. Data for WT, ent3 ${Delta}$ and ent5 ${Delta}$ cells were from a previous experiment. n=2, Bars indicate range of values. Strains used in this figure were BY4742 (WT) and derivates: syn8 ${Delta}$ , JZY1, JZY3, JZY2, ent3 ${Delta}$ , ent5 ${Delta}$ , BKY13