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Figure 5


Fig. 5. Pep12p did not colocalize with Ent3p in wild-type cells and was more diffuse in ent3{Delta} ent5{Delta} and syn8{Delta} ent3{Delta} ent5{Delta} cells. Wild-type (SEY6210, A-C; BY4742, D,G), ent3{Delta} ent5{Delta} (BKY13, E, H) and syn8{Delta} ent3{Delta} ent5{Delta} (JZY2, F,I) cells were grown at 30°C and immunofluorescence was done using antisera against Pep12p (A,C-F) and Ent3p (B,C). Cells grown at 30°C were incubated with FM4-64 for 5 minutes to stain endosomal structures (G-I). The staining pattern was unchanged, indicating that endosomes were intact in mutant cells. Bar, 5 µm.