Fig. 6. 185/333⁺ cells appear in small aggregations of coelomocytes. (A) Small aggregations of live cells were imaged with digitally enhanced phase-contrast microscopy (arrow) which shows that these aggregates are present in the living state. In addition to the phagocytes in A, there are red and colorless spherule cells and vibratile cells. (B) Aggregates labeled for 185/333 proteins (red), actin (green) and DNA (blue) shows that most of the cells in the aggregate are 185/333⁺. In this case, the aggregate is surrounded by type 2 (polygonal) coelomocytes, of which one has 185/333⁺ perinuclear vesicles (arrow). Bars, 10 µm.