Fig. 7. Numbers of coelomocytes and the frequency of 185/333⁺ cells increase after challenge with LPS. (A) Total coelomocyte counts were taken at various times after sea urchins were injected with LPS (n=4, bars indicate s.d.). (B) Injection of LPS increased the relative frequency of all 185/333⁺ coelomocytes (% of total coelomocytes) in CF collected at various times after LPS injection (n=4, bars indicate s.d.). (C) Coelomocytes and 185/333⁺ coelomocytes from adult sea urchins (n=7) were counted separately before (white bar) and after (black bar) administering two separate injections of LPS (1 µg LPS/ml CF) at 0 hours and 24 hours. Small and polygonal phagocytes are shown as the percentage of total coelomocytes. The 185/333⁺ cells are shown as the percentage of small or polygonal phagocytes. Asterisks indicate significant differences (see text) between pre-challenged and LPS challenged sea urchins.