Fig. 5. Silencing of NBS1 increases formation of rAAV foci. (A) Western blotting showing the levels of NBS1 in HeLa cells after treatment with siRNAs against NBS1 (siNbs1) or luciferase (siLuc) at 60 hours after siRNA transfection (corresponding to 24 hours p.i.). The western blotting against tubulin was used as a loading control. (B) Representative images of cells treated with anti-NBS1 or anti-Luc siRNAs followed by transduction with AAV-LacO.14. The images were taken at 24 hours p.i. without HU treatment. (C) Percentage of HeLa cells with detectable AAVLacO.14 foci after silencing of NBS1. Cells were evaluated for the presence of rAAV foci 16 and 24 hours after transduction, in the presence or absence of HU treatment, as indicated. (D) Number of AAVLacO.14 foci per cell after silencing of NBS1. Conditions were the same as in C.