Fig. 2. Effect of EPHA2 expression on level and phosphorylation of cadherin or catenin proteins and the composition of adherens junction complexes. (A) Total levels of E-cadherin, p120 catenin, β-catenin and -catenin were assayed by western blot analysis. No significant differences in expression of these proteins were detected in the respective cell types in the presence or absence of ephrin-A1 stimulation. (B) Association of the catenin proteins to E-cadherin was assessed by coimmunoprecipitation of p120 catenin, β-catenin or -catenin with E-cadherin. The ability of these proteins to associate with E-cadherin was unchanged regardless of ephrin-A1 stimulation. (C-F) E-cadherin is localized at cell-cell junctions in confluent MCF-10A, vector control, MCF-10A-EPHA2 and MCF-10A-C cells. (G) Association of catenin proteins with E-cadherin was assayed by coimmunoprecipitation in the presence of normal or low Ca²⁺ medium. Tyrosine phosphorylation of cadherin or catenin proteins was determined by western blot analysis.