Fig. 5. Effects of IQGAP1 depletion on EXO70. (A) Depletion of IQGAP1 with targeted RNAi. A representative western blot with the indicated antibodies from equal amounts of total protein from HeLa cells transfected at 100 nM with either control RNAi (first lane) or IQGAP1-RNAi (second lane). (B) Effects of IQGAP1 depletion on EXO70 localization. Upper panels: co-localization of IQGAP1 with EXO70 in control HeLa cells. Fixed cells were double-stained with antibodies for IQGAP1 (Texas red) and EXO70 (Alexa-Fluor-488, green). Lower panels: localization of EXO70 (right panel) in HeLa cells that were depleted of IQGAP1 (left panel).