Fig. 4. Distal tip localization of Ift88p-GFP in the IFT122A knockout cells. (A) Strategy to introduce IFT88-GFP constructs into the wild-type cells or IFT122A knockout cells. (B) Ift88p-GFP fluorescence in the wild-type cells. (C) Enlarged image from the boxed region in B. Ift88p-GFP localized to the cilia in oral apparatus (OA), along the somatic cilia (arrowheads) and to the basal body rows (arrows). (D) Ift88p-GFP fluorescence in the IFT122A knockout cells. (E) Ift88p-GFP fluorescence merged with DIC images. (F,G), Enlarged images from the boxed region in E. In the IFT122A knockout background, Ift88p-GFP became enriched at the distal tips of oral cilia (OA) and somatic cilia (arrowhead). Scale bars, 10 µm.