Fig. 3. Nuclear:cytoplasmic (N:C) ratios of quantitative immunofluorescence staining demonstrate comparative nuclear translocation of NF-B subunits. p52 and RelB have high N:C ratios (>1) irrespective of vFLIP, but RelA/p65 shows low N:C ratios (<1) that increase significantly in cells transduced with the vFLIP_eGFP vector. Box-and-whisker plots show median, standard error and range of data from analysis of five high-power-field images. Statistical analysis was performed using the Mann-Whitney U test.