Fig. 2. Distribution of brush border hydrolases in wt and gal3^–/– mice. Transverse sections of small intestines of wt (gal3^+/+) and gal3-null mutant (gal3^–/–) mice were stained with antibodies against LPH, DPPIV, SI (Alexa Fluor 488, green) and APN (Alexa Fluor 546, red). Cross sections were taken from the upper third of the villi. Higher magnifications of two adjacent brush border domains are shown in the bottom half of each panel. Hoechst 33342 nuclear counterstaining is blue. Scale bars: 20 µm.