Fig. 4. Distribution of giantin, Lamp1, Rab8 and Rab11 in enterocytes from wt and gal3^–/– mice. Transverse sections of small intestines of wt (gal3^+/+) and gal3-null mutant (gal3^–/–) mice were stained with antibodies against giantin (Alexa Fluor 546; red), Lamp1 (Alexa Fluor 488; green), Rab8 (Alexa Fluor 488; green) and Rab11 (Alexa Fluor 546; red). Cross sections were taken from the upper third of the villi. Higher magnifications of two adjacent brush border domains are shown in the bottom half of each panel. Hoechst 33342 nuclear counterstaining is blue. Scale bars: 20 µm.