Fig. 6. Localisation of LPH in wt and gal3^–/– mouse enterocytes. Fixation of the tissues samples was performed as described by Elsasser et al. (Elsasser et al., 1993). Antibody binding was visualised by incubation with a monoclonal anti-LPH antibody and 10 nm immunogold-conjugated goat anti-mouse IgG antibody, as indicated by arrowheads. (A) In the absence of anti-LPH, no staining was observed. (B,C) Antibody staining of brush borders from wt mice. Enterocytes from gal3^–/– mice revealed intense LPH labelling of accumulated intracellular vesicles as well as brush border staining (B). This is also visible in the higher magnifications of intracellular vesicles (G,H), whereas minor intracellular LPH staining could be detected in enterocytes from wt mice (D,E). Scale bars: 2 µm.