Fig. 7. Distribution of basolateral and apical markers. Cross-sections of small intestine villi of wt (gal3^+/+) and gal3-null mutant (gal3^–/–) mice stained with antibodies against apical markers villin (Alexa Fluor 488, green), β-actin and ezrin (Alexa Fluor 546, red), and with the basolateral marker E-cadherin. Cross sections were taken from the upper third of the villi. Higher magnifications are shown in the bottom half of each panel. Hoechst 33342 nuclear counterstaining is blue. Scale bars: 20 µm.