Fig. 10. Stable expression of SrcDN prevents TH- or TN-induced downregulation of TG and E-cadherin. (A) Western blots of total protein extracts from PC pSG5 cells, clones (Cl.) 12, 20 and 15 starved for 48 hours in hormone- and serum-free medium, then vehicle treated or treated with 5 nM EGF for 5 minutes. Filters were probed with antibodies against phosphorylated Src (p-Src; Tyr416) or total Src (c-Src). (B) Western blots of total protein extracts from PC pSG5 cells and clones 12, 15, and 20 vehicle treated or treated with 0.5 µg/ml TN for 30 minutes, followed by 24 hours in medium without TN. Filters were probed with antibodies against TG, E-cadherin and β-actin.