Fig. 3. Time-course of total protein and thyroglobulin (TG) synthesis following ER stress. (A) PC Cl3 cells were treated with 0.5 µM TH for the times indicated and labeled for 5 minutes with [³⁵S]-met/cys mix. Cell lysates were subjected to SDS-PAGE. (B) Values shown represent the mean (±s.d.) of three independent experiments. (C) PC Cl3 cells were treated and labeled as in (A), TG was immunoprecipitated and subjected to SDS-PAGE. (D) Values shown represent the mean (±s.d.) of three independent experiments.