Fig. 3. Co-localization of CD147 and NOD2 at the membrane of epithelial and myelomonocytic cells. (A) HeLaS3 cells were transiently transfected with GFP-NOD2 (green) and stained for CD147 (red) and DAPI as a DNA counterstain (blue). (B) Transfected cells were infected with L. monocytogenes (MOI=100) and fixed after 1 hour. In the basal as well as in the infected state, NOD2 and CD147 colocalize in the membrane compartment. An accumulation of both NOD2 and CD147 in areas of early bacterial invasion is detectable (open arrowheads and inset in B). (C) Indirect immunofluorescence micrographs of PMA-differentiated THP1 myelomonocytic cells. Staining with specific antibodies against endogenous NOD2 (red channel) and CD147 (green channel) shows a partial colocalization at the cell membrane (arrowheads in merged image). Images are representative of at least three independent experiments. Original magnification: 100x.