Fig. 7. CD147 does not influence expression of host cell entry factors. (A) Gentamicin protection assays with Listeria monocytogenes wild-type (L.m.) or mutant strains deficient in either internalin A or internalin B (InlA, InlB) were performed with HEK293^NOD2 cells as described in Materials and Methods. Deletion of InlB markedly reduced the ability of Listeria to invade into HEK293 cells, whereas deletion of InlA only had a marginal effect. Relative invasion is shown as percentage of CFUs compared to Listeria wild-type strain; data represent the mean ± s.d. (n=3); ^**P<0.01. (B) Cell surface expression of c-Met and E-cadherin. Cells stably transfected with pSUPER constructs silencing CD147 expression (red) or transiently transfected with EGFP-CD147 (green) were assayed for cell surface expression of Met (upper panel) or E-cadherin (lower panel) as described. Untransfected cells were used as positive control (black), cells treated with secondary antibody alone were used as specificity control (gray curve). Neither overexpression nor knock-down of CD147 influenced Met or E-cadherin expression level.