Fig. 1. Detection of 9 integrin subunit in a variety of tissues isolated from adult rat. The isolated tissues were cut into small pieces and lysed. Lysates were separated under reduced conditions by SDS-PAGE (7.5% gel) and electrotransferred into a PVDF membrane. Membrane was incubated with anti-9 polyclonal antibody against the cytoplasmic domain of integrin (A) or with IgG isolated from pre-bleed serum of the same rabbit (B). The bands were visualized using a chemiluminescent western detection kit. The molecular mass markers are indicated by arrows. Lane a, lysate of mock-SW480 cells – negative control; lane b, lysate of 9SW480 cells – positive control; lane c, vena cava; lane d, aorta; lane e, brain; lane f, kidney; lane g, heart; lane h, lung; lane i, liver.