Fig. 6. Chemotaxis of 9SW480 cells to mNGF. (A) Chemotaxis was assessed in a Boyden chamber with fluoroblock membranes (8.0 µm). Calcein-labeled cells were added to the upper chamber of the membrane with immobilized collagen IV, and mNGF was added to the lower chamber as a chemoattractant. Random migration is indicated by ; , 2.5 µg/ml; , 5 µg/ml; , 10 µg/ml; , 20 µg/ml. (B) Inhibition of chemotaxis of 9SW480 cells to mNGF (5 µg/ml). The measurement of migration was assessed after 4 hours. Random migration is represented by bar a; control migration to mNGF without inhibitors, bar b; inhibition by control mouse IgG (10 µg/ml), c; by Y9A2 (10 µg/ml), d; by disintegrins eristostatin (1 µM), e: and by VLO5 (1 µM), f. Error bars indicate s.d. from three separate experiments. ^*Significant difference (P<0.001) in relation to the group treated with 5 µg/ml of mNGF alone (b).