Fig. 7. Effect of NGF and 9β1 integrin inhibitors on cell proliferation in BrdU assay. (A) 9SW480 cells () or mock-SW480 cells () were grown in 96-well plates to 70% confluence and starved for 48 hours in FBS-free medium. Cells were not treated or treated with different concentrations of hrNGF in serum-free medium for 48 hours. BrdU color development assay was performed according to the manufacturer's instruction (Roche). Error bars indicate s.d. from triplicate experiments. (B) Effect of 9β1 integrin and TrkA inhibitors on mNGF-induced proliferation of 9SW480 cells. Cells were non-treated (a) or treated with 1 µg/ml of mNGF (b) in the presence of 10 µg/ml control mouse IgG (c) 10 µg/ml Y9A2 (d), 1 µM disintegrins eristostatin (e) and VLO5 (f), 10 µg/ml control rabbit serum (g), 10 µg/ml anti-TrkA polyclonal serum (h), or 50 µg/ml vincristine (i). Error bars indicate s.d. from three separate experiments. ^*Significant difference (P<0.001) in relation to the group treated with 1 µg/ml of mNGF alone (b).