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Figure 1


Fig. 1. POPX interacts with mDia1 in the presence of RhoAV14. (A) A construct of flag-tagged full-length mDia1 was co-transfected with GFP-POPX2 and other cDNA constructs (HA-RhoAV14 or GST-SRC) into COS7 cells. Flag-immunoprecipitated proteins from cell lysates were analyzed by SDS-PAGE and western blotting using anti-GFP antibodies to detect co-precipitated POPX2. Lane 1, mDia-FL + POPX2; 2, mDia-FL + POPX2 + GST-SRC; 3, mDia-FL + POPX2 + HA-RhoV14; 4, mDia-FL + POPX2 + HA-RhoV14 + GST-SRC. (B) Schematic of the various mDia constructs. (C) GST-POPX2 was transfected with different Flag-tagged mDia1 constructs into COS7 cells. Cell lysates were incubated with glutathione beads. The bound protein was eluted and analyzed by SDS-PAGE and western blotting using anti-Flag antibodies to detect co-purification of mDia molecules. Lane 1, POPX2 + mDia-FL; 2, POPX2 + mDia-DN; 3, POPX2 + mDia-{Delta}N1; 4, POPX2 + mDia-{Delta}DAD; 5, POPX2 + mDia-GBD.